Multiplexed Three-Dimensional Protein Mapping
- Type: Conference Presentation
- Conference Type: AIChE Annual Meeting
- Presentation Date: November 9, 2021
- Duration: 18 minutes
- Skill Level: Intermediate
- PDHs: 0.50
Proteomic imaging via immunohistochemistry is another powerful tool to map the cellular identity within tissue context. Proteomic imaging relies on the specific binding between antibody and antigen to detect protein expression and post translational modifications that have direct indications of the cellular identity. With a large pool of robust antibodies developed over decades, most types of tissues, including postmortem human brain samples from brain banks, can be labeled and analyzed based on the expression of proteins of interest or pathological markers. However, the throughput of immunohistochemistry is still limited by the spectral overlap between dyes and the cross-reactivity of antibodies imposed by host animals.
Here, we introduce a highly scalable, multiplexed immunostaining method that enables the extraction of cellular signature based on the simultaneous reading of many proteins. We demonstrated that proteins can be analyzed in a multiplexed and high throughput manner in postmortem human brain tissue sections.
|AIChE Member Credits||0.5|
|AIChE Graduate Student Members||Free|
|AIChE Undergraduate Student Members||Free|