Characterizing the Hydrophobic Interactions of Fusion Peptides of Coronaviruses Using Single-Molecule Force Measurements
- Type: Conference Presentation
- Conference Type: AIChE Annual Meeting
- Presentation Date: November 7, 2021
- Duration: 15 minutes
- Skill Level: Intermediate
- PDHs: 0.50
This presentation will focus on results obtained with two peptide sequences:
First, we will describe single molecule force measurements of a short peptide sequence from FP1 that contains LLF. We will use the measurements to address several key questions: 1) Does LLF play a central role in mediating hydrophobic interactions of SARS-2 FP1 with model surfaces? 2) Is there a difference in the hydrophobic interaction strength between SARS-2 and MERS, where LLF is flanked by distinct residues?
Second, we will report single molecule force measurements using a peptide sequence that spans FP1 and FP2, presenting four potential Ca2+ binding sites. We will use the measurements to address the question of whether or not binding of Ca2+ to FP impacts hydrophobic forces encoded by the sequence. The results of our single-molecule force measurements will be placed into the context of independent measurements of fusion peptide activity.
|AIChE Member Credits||0.5|
|AIChE Graduate Student Members||Free|
|AIChE Undergraduate Student Members||Free|