Replacing Your Plasmid Construction by OriCiro™ Cell-Free Cloning System | AIChE
Sponsor: OriCiro Genomics | This webinar is sponsored by OriCiro Genomics and reflects their views, opinions, and insights. Attendance is free.

Replacing Your Plasmid Construction by OriCiro™ Cell-Free Cloning System

In this webinar, Prof. Su’etsugu will talk about a novel tool kit for life science researchers, “OriCiro™ Cell-free Cloning System.” Many life science laboratories perform a conventional DNA cloning relying on E. coli which has been developed almost fifty years ago. The E. coli cloning is time-consuming, labor-intensive and has a limitation in efficiency and applicable size. Some DNA sequences are difficult to get a clone due to a cell-toxicity. How can you streamline your cloning workflow and widen the scope of your research?

The OriCiro™ Cell-free Cloning System consists of two enzymatic reactions, Assembly and Amplification. The Assembly reaction allows seamless assembly of multiple overlapping DNA fragments (>50 fragments) in a one-step reaction. The assembly products including assembly intermediates can be added directly to the Amplification reaction to amplify selectively your target circular DNA like a plasmid. After the reaction (within several hours), you’ll get a high concentration of the circular DNA (up to 100 ng/µL) with a great purity. Because a whole replication cycle of the E. coli chromosome is reconstituted in vitro using 26 purified proteins, the Amplification reaction has a unique feature in amplification fidelity (a single error/100-Mb/cycle) and amplification size (up to 50-kb in the commercial version). Any sequences including GC-rich or cell-toxic sequences are applicable.

You’ll learn the OriCiro™ Cell-free Cloning System as a powerful, rapid and easy tool-kit for your DNA construction/amplification in the era of synthetic biology.

Sponsored webinars bring technical information from reputable firms and suppliers to AIChE members. The content reflects the views, opinions, and recommendations of the sponsoring organization. AIChE does not warrant or represent, expressly or by implication, the correctness or accuracy of the content of the information presented. As between (1) the AIChE, the presenter and author(s) of this work, their employers, and their employers' officers and directors, and (2) the user/viewer of this work, the user/viewer accepts any legal liability or responsibility whatsoever for the consequence of its use or misuse. Contact information for attendees of this webinar, including email address, will be shared with the sponsoring firm. You will always have the opportunity to unsubscribe from email from that organization.

  • AIChE Pro Members - Free
  • AIChE Graduate Student Members - Free
  • AIChE Undergraduate Student Members - Free
  • AIChE Explorer Members - $0.00
  • Non-Members - $0.00
Do you already own this? Log In for instructions on accessing this content.
  • Source:
  • Language:
  • Skill Level:
  • Duration:
    1 hour
  • PDHs: