Metabolic Engineering relies on the Design-Build-Test cycle. This cycle includes technologies like mathematical modeling of metabolism, genome editing and advanced tools for phenotypic characterization. In recent years there have been advances in several of these technologies, which has enabled faster development of metabolically engineered strains that can be used for production of fuels and chemicals.
The yeast Saccharomyces cerevisiae is widely used for production of fuels, chemicals, pharmaceuticals and materials. Through metabolic engineering of this yeast a number of novel industrial processes have been developed over the last 10 years. Besides its wide industrial use, S. cerevisiae also serves as an eukaryal model organism, and many systems biology tools have therefore been developed for this organism. These tools can be used for detailed phenotypic characterization as well as for metabolic design.
In this lecture it will be demonstrated how the Design-Build-Test cycle of Meta-bolic Engineering has allowed for development of yeast cell factories for production of a range of different fuels and chemicals. Some examples of different technologies will be presented together with examples of metabolic engineering designs, in particular for development of platform strains that can be used for production of a fatty acid derived products, e.g. fatty alcohols and alkanes. In the presentation it will also be shown that the Design-Build-Test cycle can be ex-panded to incorporate adaptive laboratory evolution to identify targets for engineering complex traits, such as improved tolerance to toxic metabolites like butanol, elevated temperatures or low pH, or when we want to engineer the highly complex protein secretory pathway.