Replacing Your Plasmid Construction by OriCiro™ Cell-Free Cloning System | AIChE
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Replacing Your Plasmid Construction by OriCiro™ Cell-Free Cloning System

Originally delivered Oct 20, 2020
Source: OriCiro Genomics | This webinar is sponsored by OriCiro Genomics and reflects their views, opinions, and insights. Attendance is free.
  • Type:
    Archived Webinar
  • Level:
    Intermediate
  • Duration:
    1 hour
  • PDHs:
    0.00

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Preview Webinar: 

In this webinar, Prof. Su’etsugu will talk about a novel tool kit for life science researchers, “OriCiro™ Cell-free Cloning System.” Many life science laboratories perform a conventional DNA cloning relying on E. coli which has been developed almost fifty years ago. The E. coli cloning is time-consuming, labor-intensive and has a limitation in efficiency and applicable size. Some DNA sequences are difficult to get a clone due to a cell-toxicity. How can you streamline your cloning workflow and widen the scope of your research?

The OriCiro™ Cell-free Cloning System consists of two enzymatic reactions, Assembly and Amplification. The Assembly reaction allows seamless assembly of multiple overlapping DNA fragments (>50 fragments) in a one-step reaction. The assembly products including assembly intermediates can be added directly to the Amplification reaction to amplify selectively your target circular DNA like a plasmid. After the reaction (within several hours), you’ll get a high concentration of the circular DNA (up to 100 ng/µL) with a great purity. Because a whole replication cycle of the E. coli chromosome is reconstituted in vitro using 26 purified proteins, the Amplification reaction has a unique feature in amplification fidelity (a single error/100-Mb/cycle) and amplification size (up to 50-kb in the commercial version). Any sequences including GC-rich or cell-toxic sequences are applicable.

You’ll learn the OriCiro™ Cell-free Cloning System as a powerful, rapid and easy tool-kit for your DNA construction/amplification in the era of synthetic biology.

Presenter(s): 

Masayuki Su'etsugu

Prof. Su'etsugu is a Chief Science Officer of OriCiro Genomics, Inc. He is also a Professor of Synthetic Biology at Rikkyo University located in Tokyo. He developed a novel synthetic biology technology for constructing and amplifying a large circular DNA such as a plasmid or genome by reconstituting a whole replication process of the E. coli chromosome in vitro (Su’etsugu et al., Nucleic Acids Res. 2017). On the basis of this technology, he co-founded the spin-off company, OriCiro Genomics, Inc in 2018.

He started his laboratory at Rikkyo University in 2013. His laboratory currently...Read more

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Sponsored webinars bring technical information from reputable firms and suppliers to AIChE members. The content reflects the views, opinions, and recommendations of the sponsoring organization. AIChE does not warrant or represent, expressly or by implication, the correctness or accuracy of the content of the information presented. As between (1) the AIChE, the presenter and author(s) of this work, their employers, and their employers' officers and directors, and (2) the user/viewer of this work, the user/viewer accepts any legal liability or responsibility whatsoever for the consequence of its use or misuse. Contact information for attendees of this webinar, including email address, will be shared with the sponsoring firm. You will always have the opportunity to unsubscribe from email from that organization.

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