Design of a Colloidal Network for Antimicrobial Peptide Protection and Delivery
- Type: Conference Presentation
- Conference Type: AIChE Annual Meeting
- Presentation Date: November 9, 2021
- Duration: 18 minutes
- Skill Level: Intermediate
- PDHs: 0.50
Here, we present an injectable and biodegradable colloidal nano-network (NN) to protect AMPs against enzymatic degradation. Positively (pNPs; ~400 nm) and negatively charged nanoparticles (nNPs; ~300 nm) were formed via complex coacervation of chitosan and dextran sulfate. The charged AMP PA-13 was encapsulated into the NPs by electrostatic complexation without the requirement of peptide chemical modifications.7 A colloidal NN was formed by simple mixing of pNPs with nNPs and rheological analysis demonstrated that the NN could flow through narrow gauge needles (shear-thin) while retaining its shape after being extruded (self-heal). This rheology enables application of the AMP-loaded NN as a cream or injectable gel. After exposure to peptidases the formed NN loaded with PA-13 eliminated most of Pseudomonas aeruginosa (Î5 log CFU/mL) while the unprotected PA-13 had no effect (Î0 log CFU/mL), demonstrating the ability of the NN to protect AMPs.
Overall, our study demonstrates that the designed NN is a potential platform for AMP protection against enzymatic degradation without negatively affecting peptide activity. Given the simplicity of the AMP inclusion into the NN, we envision that this platform could encapsulate a variety of charged peptides through electrostatic complexation.
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