Metabolic Modulation in Response to Chemical-Induced Signal Transduction in Chlamydomonas Reinhardtii

Chlamydomonas reinhardtii is a eukaryote algae model system for photosynthetic organisms. Chlamydomonas is suitable for studying complicate signaling pathways that are evolutionary or clinically important (e.g. target of rapamycin, jasmonate, and salicylic acid signaling pathways). In current report, we elicitated signal transduction pathways of Chlamydomonas by various chemicals, and monitored physiology-relevant metabolic modulation using metabolomic approaches. Comprehensive quantitative metabolomic tools have been used for reconstructing the dynamic network in living cells in time-dependent and dose-responsive metabolic changes by applying analytical chemistry and informatics tools in order to provide a multi-dimensional gateway to cellular responses that is otherwise inaccessible to genomics.