Technical Program | AIChE

Technical Program

Technical Program for the 2022 conference on CRISPR technologies.

5th International Conference on CRISPR Technologies

Day 1 - Monday, October 31, 2022

All Pacific Standard Time  
7:30 AM 4:00 PM Registration
8:00 AM 8:30 AM Welcome & Breakfast
8:30 AM 11:00 AM Phage Engineering 

Chair: Gaetan Burgio, Australian National University
8:30 AM 8:33 AM Welcome Remarks by Chairs: Gavin Knott, Audrey Lapinaite, James Nuñez
8:33 AM 8:35 AM Phage Engineering Introduction, Gaetan BurgioAustralian National University
8:35 AM 9:25 AM KEYNOTE: On to the next decade of the CRISPR craze: genome editing at scale across the tree of life, Rodolphe BarrangouNCSU
9:25 AM 9:45 AM Strategies for phage engineering using a Type III-A CRISPR-Cas system, Asma Hatoum Aslan, University of Illinois Urbana-Champaign
9:45 AM 9:50 AM 5 minute stretch break
9:50 AM 10:10 AM Genome-wide gene essentiality mapping of phages using CRISPRi technology, Vivek MutalikLBNL
10:10 AM 10:30 AM Brady Cress & Ben AdlerIGI
10:30 AM 11:00 AM Morning Coffee Break
11:00 AM 12:20 PM Agricultural and ecological applications of CRISPR 

Chair: Patrick Shih, UC Berkeley/JBEI
11:00 AM 11:05 AM Agricultural and ecological applications of CRISPR Introduction, Patrick ShihUC Berkeley/JBEI
11:05 AM 11:25 AM Improving plant nitrogen use efficiency via CRISPR/Cas9 genetic engineering, Gozde DemirerCaltech
11:25 AM 11:45 AM CRISPR-Combo Enables Speed Breeding and Improved Tissue Culture in Plants, Yiping QiUniversity of Maryland
11:45 AM 12:05 PM Seed development: from embryo initiation to engineering clonal seeds, Imtiyaz KhandayUC Davis
12:05 PM 12:20 PM Versatile Genome Manipulation Using Reverse Transcriptase and Type V CRISPR-Cas Systems, Y. Bill Kim, Pairwise Plants
12:20 PM 1:20 PM Lunch Workshop-Cellecta 

Flexible and Scalable Genetic Screens for Discovery and Characterization of Novel Therapeutic Targets, Paul DiehlCellecta
1:20 PM 2:40 PM Delivery of CRISPR-based tools 

Chairs: Ross Wilson, UC Berkeley & Peter Cameron, Spotlight Therapeutics
1:20 PM 1:25 PM Delivery of CRISPR-based tools Introduction, Ross WilsonUC Berkeley & Peter CameronSpotlight Therapeutics 
1:25 PM 1:45 PM Peptide-enabled CRISPR enzyme delivery for versatile ex vivo T cell engineering and in vivo editing of the brain, Ross WilsonUC Berkeley
1:45 PM 2:05 PM Erik SontheimerUniversity of Massachusetts
2:05 PM 2:25 PM Romu CorbauGenEdit
2:25 PM 2:40 PM Large Knock-in in Primary T Cells with Optimized Cas9 HDR Methods and Design, Bernice ThommandruIntegrated DNA Technologies
2:40 PM 3:10 PM Afternoon Coffee Break
3:10 PM 4:15 PM Social, ethical, and economic implications of CRISPR-based technologies 

Chair: Gaetan Burgio, Australian National University
3:10 PM 3:15 PM Social, ethical, and economic implications of CRISPR-based technologies Introduction, Gaetan BurgioAustralian National University
3:15 PM 3:35 PM

Getting Creative with CRISPR Outreach and Scientific Publishing, Megan Hochstrasser, IGI Berkeley

3:35 PM 3:55 PM Unresolved technical challenges with human germline gene-editing, Paul KnoepflerUC Davis
3:55 PM 4:15 PM Ethical, Legal, and Social Issues in Three Applications of CRISPR Technologies, Hank GreelyStanford University
4:15 PM 4:25 PM Danaher Corporation, Aldevron, and IDT Cocktail Reception Introduction 
4:25 PM 5:25 PM Danaher Corporation, Aldevron, and IDT Cocktail Reception
4:30 PM 5:15 PM Poster Session 1

Day 2 - Tuesday, November 1, 2022

All Pacific Standard Time  
7:30 AM 4:00 PM Registration
8:00 AM 8:30 AM Welcome & Breakfast
8:30 AM 10:35 AM CRISPR-Cas Biology 

Chair: Audrey Lapinaite, Arizona State Univeristy & Gavin Knott, Monash University
8:30 AM 8:35 AM CRISPR-Cas Biology Introduction, Audrey LapinaiteArizona State Univeristy & Gavin KnottMonash University
8:35 AM 8:55 AM Consequences of Cas effector specificity in bacteriophage evolution, Dipa SashitalIowa State University
8:55 AM 9:15 AM Structures of the holo CRISPR RNA-guided transposon integration complex, Elizabeth KelloggCornell University
9:15 AM 9:20 AM 5-minute Stretch Break
9:20 AM 9:40 AM Dynamics and mechanisms of CRISPR-Cas9 through the lens of computational method, Giulia PalermoUC Riverside
9:40 AM 10:00 AM Type III CRISPR-Cas provides resistance against nucleus-forming jumbo phages via abortive infection, Peter FineranUniversity of Otago
10:00 AM 10:20 AM Delivering Cas9 mRNA to tissues in mice using the SEND system, Gurumurthy Channabasavaiah, University of Nebraska Medical Center
10:20 AM 10:35 AM Novel Base Editors Engineered from Cytosine Deaminases and CRISPR Effectors Uncovered from Diverse Environments, Cindy CastelleMetagenomi
10:35 AM 11:05 AM Morning Coffee Break
11:05 AM 12:25 PM Genome Editing and DNA Repair 

Chair: Julian Grünewald, Technical University of Munich
11:05 AM 11:10 AM Genome Editing and DNA Repair Introduction, Julian GrünewaldTechnical University of Munich
11:10 AM 11:30 AM CRISPR-based approaches to study the DNA damage response, Alberto CicciaColumbia University Medical Center
11:30 AM 11:50 AM Therapeutic Base and Prime Editing in Fanconi Anemia Hematopoietic Stem and Progenitor Cells, Paula RioCIEMAT Madrid
11:50 AM 12:10 PM Nucleic acid sensing and innate immunity in gene engineering, Anna Kajaste-RudnitskiSan Raffaele-Telethon Institute for Gene Therapy
12:10 PM 12:25 PM Marker-free coselection for successive rounds of prime editing in human cells, Yannick Doyon, Centre de recherche du CHU de Québec – Université Laval
12:25 PM 1:25 PM Lunch Workshop - Danaher Corporation, Aldevron, and IDT

Delivering the Next Generation of Breakthrough Genomic Medicines, Sadik KassimDanaherTom FotiAldevron & Mark BehlkeIDT
1:25 PM 2:45 PM Clinical and biomedical applications of CRISPR 

Chair: Marco Herold, WEHI
1:25 PM 1:30 PM Clinical and biomedical applications of CRISPR Introduction, Marco HeroldWEHI
1:30 PM 1:50 PM CRISPR Cures: an Approach to the "Two Last Miles" Problem, Fyodor UrnovUC Berkeley
1:50 PM 2:10 PM Correcting genetic diseases by in vivo prime editing, Gerald Schwank,  ETH Zurich, Switzerland
2:10 PM 2:30 PM Systematically deciphering the roles of E3-ligase family members in inflammatory responses with massively parallel Perturb-seq, Katie Geiger SchullerGenentech
2:30 PM 2:45 PM CRISPR-Cas-Mediated Ablation of the Negative T Cell Regulators Enhances the Therapeutic Efficacy of CAR T Cells, Giedre KrenciuteSt Jude Children's Research Hospital
2:45 PM 3:15 PM Afternoon Coffee Break
3:15 PM 4:55 PM Genome Screening & Discovery

Chair: James Nuñez, UC Berkeley
3:15 PM 3:20 PM Genome Screening & Discovery Introduction, James NuñezUC Berkeley 
3:20 PM 3:40 PM Next-generation CRISPR screening platforms for insights into human disease biology, Laralynne PrzybylaUCSF/LGR
3:40 PM 4:00 PM Defining Cellular Barriers to HIV infection through HIV-CRISPR Knockout Screening, Molly OhainleUC Berkeley
4:00 PM 4:20 PM Jain Piyush, University of Florida
4:20 PM 4:40 PM Delivering insights into organ homeostasis and regeneration through in vivo genome-wide screens, Kristin KnouseMIT
4:40 PM 4:55 PM The Crisprverse: A Comprehensive Ecosystem for the Design of CRISPR Guide RNAs across Nucleases and Technologies, Jean-Philippe FortinGenentech
4:55 PM 5:15 PM Sponsored Talk: Leveraging industrialized CRISPR technologies to build an impactful academic-industry partnership, Kevin Holden, Synthego
4:15 PM 5:20 PM Banquet Dinner Reminder
6:00 PM 9:00 PM BANQUET DINNER - 10 Year Anniversary of CRISPR Engineering 

Day 3 - Wednesday, November 2, 2022

All Pacific Standard Time  
7:30 AM 4:00 PM Registration
8:00 AM 8:30 AM Welcome & Breakfast
8:30 AM 11:50 AM CRISPR-Cas Microbiome Editing

Chair: Ben Rubin, UC Berkeley
8:30 AM 8:35 AM CRISPR-Cas Microbiome Editing Introduction, Ben RubinUC Berkeley
8:35 AM 9:25 AM KEYNOTE: The Past, Present and Future of CRISPR, Jennifer DoudnaUC Berkeley
9:25 AM 9:45 AM A genetic approach to study microbe-host interaction at the molecular level, Chun-Jun GuoCornell
9:45 AM 10:05 AM Highly efficient bacterial conjugation enables precise Cas9-mediated gut microbiome editing, Kevin NeilTatum
10:05 AM 10:35 AM Morning Coffee Break
10:35 AM 10:55 AM Carlotta RondaColumbia 
10:55 AM 11:15 AM Connor TsuchidaUC Berkeley 
11:15 AM 11:35 AM Engineering Living Medicines for Chronic Diseases, Will DeloacheNovome
11:35 AM 11:50 AM A Randomized Multiplex CRISPR Interference Tool to Systematically Screen Groups of Genes for Synthetic Lethality, Nicole EllisNational Institutes of Health
11:50 AM 11:55 AM 5-minute Stretch Break
11:55 AM 2:40 PM CRISPR-Cas Biology (Second half)

Chair: Audrey Lapinaite, Arizona State University & Gavin Knott, Monash University
11:55 AM 12:00 PM CRISPR-Cas Biology (Second half) Introduction, Audrey Lapinaite, Arizona State University & Gavin KnottMonash University
12:00 PM 12:20 PM R-loop formation in CRISPR-Cas(9) effector complexes David W. TaylorUniversity of Texas at Austin
12:20 PM 12:40 PM Craspase is a CRISPR RNA-guided, RNA-activated protease, Ailong KeCornell University
12:40 PM 1:00 PM Pulling the plug on bacteriophage infection: the pore behavior of the CRISPR-Cas13-activated membrane protein CRISPR-Csx28, Mitchell R. O'ConnellUniversity of Rochester
1:00 PM 2:00 PM Lunch Workshop-MilliporeSigma

The Foundation and the Bridge: Next-Generation CRISPR Tools for Target Identification, Validation, and Translational Research, Zulfiquer HossainMilliporeSigma
2:00 PM 2:20 PM Cas11 enables genome engineering in human cells with compact CRISPR-Cas3 systems, Yan ZhangUniversity of Michigan
2:20 PM 2:40 PM Dual strategies for CRISPR-Cas12 inactivation in bacteria, Nicole D MarinoUniversity of California, San Francisco
2:40 PM 2:45 PM 5-minute Stretch Break
2:45 PM 4:00 PM Mammalian Synthetic Biology

Chair: Luke Gilbert, UCSF
2:45 PM 2:50 PM Mammalian Synthetic Biology Introduction, Esther TakMGH
2:50 PM 3:10 PM Development of compact transcriptional repression and activation tools using high-throughput measurements in different genomic and cell contexts, Lacra BintuStanford University
3:10 PM 3:30 PM Cas9-mediated tagging of endogenous loci using HITAG, Alejandro ChavezUniversity of California San Diego
3:30 PM 3:45 PM CasTuner: A Degron-Based CRISPR Toolkit for Analog Tuning of Gene Expression, Gemma NovielloMPI for Molecular Genetics
3.45 PM 4:00 PM CRISPR-Based Single-Layer AND and NAND Logic Gates for Complex Genetic Circuit Construction in Yeast, Emily CliffUniversity of Washington
4:00 PM 4:45 PM Afternoon Coffee Break + Poster Session 2
4:45 PM 6:00 PM Biotechnology & Nanotechnology Applications (Beyond CRISPR) 

Chair: Gavin Knott, Monash University
4:45 PM 4:50 PM Biotechnology & Nanotechnology Applications (Beyond CRISPR) Introduction, Gavin KnottMonash University
4:50 PM 5:10 PM Lucas HarringtonMammoth Biosciences
5:10 PM 5:30 PM Discovery of type V-K CRISPR-associated transposases from metagenomic mining, Kyle WattersArbor Biotechnologies
5:30 PM 5:45 PM Expanding the genome editing toolbox with Metagenomics, Gregory CostMetagenomi 
5:45 PM 6:00 PM Engineered LwaCas13a with Enhanced Collateral Activity for Nucleic Acid Detection, Xue GaoRice University
6:00 PM 6:10 PM Closing Remarks by Chairs: Gavin Knott, Audrey Lapinaite, James Nuñez