Microbial and Human Multiplex Genome Engineering & Selection

Microbial and Human Multiplex Genome Engineering & Selection
Sunday, January 13, 2013, 5:45pm-6:45pm EST

 

George Church


Professor of Genetics, Harvard Medical School, Boston, MA
Director of Synthetic Biology, Wyss Institute for Biologically Inspired Engineering, Boston, MA
Director of NIH-Center of Excellence in Genomic Science and DOE-GTL Genomics Centers

Genome Engineering requires: 1) bio computer aided design tools; 2) raw DNA synthesis at high efficiency ( 100kb/$) and accuracy (99.98%).  3) multiplex insertion of such DNA into genomes at 5 or more per cell at once and billions of genomes per day;  4) "Sensor-selector" circuits which can keep up with the design and genome generation tools.  Libraries of 10,000 or more genetic constructs can be made inexpensively using oligonucleotide chips. Multiplex Automated Genome Engineering (MAGE) enabled the first Genomically Recoded Organism designed and shown to have new desired functionality (dedicated codon for novel amino acids, genetic isolation and virus resistance).  A highly modified CRISPR Cas9 plus guide-RNA system  greatly accelerates progress toward human MAGE. 
 

George Church

George is Professor of Genetics at Harvard Medical School and Professor of Health Sciences and Technology at Harvard and the Massachusetts Institute of Technology. He is Director of a U.S. Department of Energy Technology Center and a National Institutes of Health Center of Excellence in Genomic Science. His 1984 Harvard PhD included the first methods for direct genome sequencing, molecular multiplexing & barcoding. His innovations have contributed to nearly all “next generation” DNA sequencing methods and companies. This plus his lab’s work on chip-DNA-synthesis, gene editing and stem...Read more

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