AdvantaFlex biopharmaceutical-grade thermoplastic elastomer (TPE), previously available only as tubing, is now used to make molded Tri-Clamp fittings, BioClosure container sealing systems, and connections such as Ys, Ts, crosses, and reducers. This allows molded manifold assemblies to be manufactured from TPE, as an alternative to silicone, and allows an entire assembly to be manufactured from a single resin, which reduces the potential for installation errors. The use of molded connections eliminates the need for barbed fittings (and their potential for leakage and contamination).