Affinity Purification of Human Immunoglobulins A, G, and M by Hexamer Peptide Ligand

Separations Division
2009 AIChE Annual Meeting
AIChE Annual Meeting
November 10, 2009 - 7:00pm
Antibody-based therapeutics account for a third of all new treatments in USA and the size of the therapeutic antibody market is projected to reach over 30 billion per year by 2010. Although most of antibodies in the market and in the clinical development are immunoglobulin-G-based drugs, more and more attention has been paid to immunoglobulins A and M for their potential as mucosal vaccines and therapeutic agents. A family of linear hexamer peptides, exhibiting the ability to recognize and bind immunoglobulin G (IgG) through its Fc portion, has been previously identified through a three-step screening of a synthetic solid phase peptide ligand library. Among them, peptide ligand HWRGWV also exhibits the ability to bind IgA and IgM with different affinities because of different structures and interactions. Chromatographic resins containing HWRGWV ligand at different peptide densities have been extensively characterized for their applicability in affinity chromatography for the downstream processing of human immunoglobulins A, G, and M. The ligand shows the ability to purify hIgA, hIgG, and hIgM from complete mammalian cell culture medium containing 10% fetal calf serum and 5% tryptose phosphate broth with high recovery and purity. The influence of the elution pH and salt concentration, and the possibility for one step purification of hIgA, hIgG, hIgM mixture were also investigated.&'
Professional Development Hours
0.4 PDHs
You will be able to download and print a certificate for these PDH credits once the content has been viewed. If you have already viewed this content, please click here to login.

Would you like to access this content?

No problem. You just have to complete the following steps.

You have completed 0 of 2 steps.

  1. Log in

    You must be logged in to view this content. Log in now.

  2. Purchase Technical Presentation

    You must purchase this technical presentation using one of the options below.
    If you already purchased this content recently, please click here to refresh the system's record of ownerships.


Credits 0.5 Use credits
List Price $25.00 Buy now
AIChE Members $15.00 Buy now
AIChE Undergraduate Student Members Free Free access
AIChE Graduate Student Members Free Free access
Related Topics: