(627g) A Platform Technology for Dynamic Control of Cell Behavior
AIChE Annual Meeting
Thursday, November 14, 2019 - 9:48am to 10:30am
Our approach is based on the use of the NanoDeg (ACS Synth Biol. 2018 Feb 16;7(2):540-552) â a bifunctional system that mediates proteasomal degradation of a cellular target with high specificity and exquisite control over rate of decay. To achieve input-dependent post-translational control of the output through the NanoDeg, feedforward loop topologies were explored and compared to conventional strategies for circuit design. iterations of mathematical modeling and experimental tests allowed defining the ideal circuit architecture for integrating the NanoDeg within orthogonal cellular networks.
This work generated a platform technology for quantitative, multiplexed profiling of gene expression signatures of the UPR with high sensitivity and dynamic resolution of the stimulus causing proteotoxic stress. This study also provides the design rules of a novel cell engineering technology for building complex genetic networks that govern highly dynamic cellular behaviors.