(162l) Hemostatic Antibacterial Adhesive Hydrogel for Sutureless Tissue Sealing
AIChE Annual Meeting
Thursday, November 19, 2020 - 8:00am to 9:00am
Materials and Methods: GelMA and ZF were synthesized as explained in our previous publications. Different concentrations of SNs (10, 20 and 30 mg/ml) and varying concentrations of ZF (0-1.5 mg/ml) were added to GelMA and hydrogels were prepared by visible-light mediated crosslinking for 4 min. Physical, chemical, antibacterial, hemostatic and adhesive properties of the hydrogels were thoroughly characterized.
Results and Discussion: These multifunctional hydrogels exhibit faster swelling and degradation, and similar or superior mechanical properties compared to GelMA. Adding 1.5 mg/ml ZF endowed ~ 93% antibacterial activity to the GelMA hydrogel (Figure 1a,b) and with 20 mg/ml SNs blood clotting time of the hydrogel increased by ~40% (Figure 1c,d). In accordance with standard burst pressure test, increasing SNs concentration improved sealing efficacy of the hydrogels, and more interestingly combination of SNs and ZF with GelMA could further enhance burst strength of the hydrogel by >1.5 fold of a stand-alone GelMA (Figure 1e,f). Furthermore, our multifunctional hydrogels were biocompatible and could significantly enhance hemostasis and showed better therapeutic impact than the commercially available Surgicel absorbable hemostat in a rat liver bleeding model.
Conclusions: The synergistic contribution of SNs and ZF nanoparticles to the adhesion, hemostatic and antibacterial properties of GelMA renders our multifunctional hydrogel suitable for sealing applications, especially for the tissues which are more prone to bleeding and bacterial infection and paves the way for the fabrication of the next generation of low cost multifunction biopolymers for sutureless sealing of tissues.