Engineering Single-Cell Bioanalytics for Discovery and Bioprocess Characterization

SBE Webinar
Originally delivered Jan 24, 2012

Discovery of new antibodies for therapeutics requires discerning tools to identify rare cells producing specific antibodies among many similar ones. Bioprocess development also relies on methods to identify optimized cell lines producing heterologous proteins. This talk will describe an approach for integrated single-cell analysis that allows high-throughput screening (10^4–10^5 cells per screen) for novel antibodies and productive clones. The approach uses a modular collection of techniques employing microfabricated arrays of subnanoliter containers to enable flexible, reconfigurable processes that facilitate the discovery of novel antibodies and evaluation of clonal lines used in manufacturing heterologous proteins. The application of this nanowell-based technology to screening hybridomas and other antibody-secreting cells will be presented. The use of the same platform to identify high-producing strains for heterologous proteins will also be presented. How these measures on clonal lines can also provide insight into the biological factors that impede the recovery of stable production lines in such processes will also be discussed.

Professional Development Hours
1.0 PDHs
You will be able to download and print a certificate for these PDH credits once the content has been viewed. If you have already viewed this content, please click here to login.
Presenter(s): 

Would you like to access this content?

No problem. You just have to complete the following steps.

You have completed 0 of 2 steps.

  1. Log in

    You must be logged in to view this content. Log in now.

  2. Purchase Webinar

    You must purchase this webinar using one of the options below.
    If you already purchased this content recently, please click here to refresh the system's record of ownerships.

Pricing

Credits 1 Use credits
List Price $99.00 Buy now
AIChE Members $69.00 Buy now
AIChE Undergraduate Student Members Free Free access
AIChE Graduate Student Members Free Free access
SBE Members Free Free access
Webinar content is available with the kind permission of the author(s) solely for the purpose of furthering AIChE’s mission to educate, inform and improve the practice of professional chemical engineering. All other uses are forbidden without the express consent of the author(s). For permission to re-use, please contact chemepermissions@aiche.org.
Related Topics: