Traditional HPLC separation techniques are not well-suited for isolating significant amounts of charge variants; larger columns do not provide the necessary resolution and analytical columns require multiple cycles and significant sample handling, which can subject the material to microbial contamination.
Displacement Chromatography is a powerful tool that is well-suited for resolving closely-related species from one another. This mode forces the components to compete with each other for binding sites, exploiting small differences between components and forcing them to self-segregate as they move down the column.
This presentation describes a displacement method for isolating sufficient amounts of charge variants to study their PK effects in animal models. It demonstrates a method capable of producing charge variant pools at >90% purity. In addition, this method is ~50x faster than the current HPLC method and reduces sample handling more than 50-fold.&'
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