Traditional HPLC separation techniques are not well-suited for isolating significant amounts of charge variants; larger columns do not provide the necessary resolution and analytical columns require multiple cycles and significant sample handling, which can subject the material to microbial contamination.
Displacement Chromatography is a powerful tool that is well-suited for resolving closely-related species from one another. This mode forces the components to compete with each other for binding sites, exploiting small differences between components and forcing them to self-segregate as they move down the column.
This presentation describes a displacement method for isolating sufficient amounts of charge variants to study their PK effects in animal models. It demonstrates a method capable of producing charge variant pools at >90% purity. In addition, this method is ~50x faster than the current HPLC method and reduces sample handling more than 50-fold.&'
Would you like to access this content?
No problem. You just have to complete the following steps.
You have completed 0 of 2 steps.
You must be logged in to view this content. Log in now.
Purchase Technical Presentation
You must purchase this technical presentation using one of the options below.
If you already purchased this content recently, please click here to refresh the system's record of ownerships.
|Credits||0.5 Use credits|
|List Price||$25.00 Buy now|
|AIChE Members||$15.00 Buy now|
|AIChE Undergraduate Student Members||Free Free access|
|AIChE Graduate Student Members||$15.00 Buy now|