(55d) Improving Filtration Performance of Virus Filters at High Product Titers
AIChE Annual Meeting
2023
2023 AIChE Annual Meeting
Separations Division
Honorary Session for Prof. Steve Cramer I (Invited Talks)
Tuesday, November 7, 2023 - 1:24pm to 1:42pm
However, the capacity and fouling behavior of virus filtration at such high product titers (30 g/L or above) can be compromised by the formation of reversible and possibly irreversible product aggregates as well as by increased product-membrane interactions. In addition, increased viscosity or concentration polarization at high product titers could also reduce the flux.
Currently, the exact fouling mechanism that leads to significant flux decay is not understood. Here mAb feed streams at high product titers were characterized and correlated with virus filtration performance. The methods used include size exclusion chromatography, SDS PAGE and UV spectroscopic measurements. In addition, mAb solubility at different buffer conditions was determined. It was found that reversible product aggregates formed at high product titers are responsible largely for the flux decay during virus filtration.