(530d) Engineering Tools to Control Individual Microbes and Microbiota without Antibiotic Resistance Genes at a Single Strain Level

Microbial biocontainment is essential for engineering safe living therapeutics [1, 2]. However, the genetic stability of biocontainment circuits is a challenge. Kill switches are among the most difficult circuits to maintain due to the evolution of escape mutants. We engineered two CRISPR-based, chemical- or temperature-inducible kill switches in the probiotic Escherichia coli Nissle and demonstrated mutationally robust biocontainment [3]. In this presentation, we will discuss our machine learning-based microbiota engineering tools that are useful to manipulate microbiota and kill pathogens at a single strain level [4]. Specifically, we will discuss the development and validation of a novel computational program, ssCRISPR, which designs strain-specific CRISPR guide RNAs (gRNAs) that can be utilized to modify complex consortia. As a proof of concept, we applied the program to two novel applications: the isolation of specific microbes from consortia through plasmid transformations and the removal of specific microbes from consortia through liposome-packaged CRISPR antimicrobials. Additionally, we will discuss antibiotic resistance gene-free plasmid systems that prevent antibiotic resistance spread via horizontal gene transfer [5]. This new technology has vast implications in designing strain-specific antimicrobials and combating the growing concern of antibiotic- and bacteriocide-resistant microbes.

1. Curr Opin Biotechnol, 2020. 66, 11-17.
2. Biotechnology Journal, 2020. 15, 1900319.
3. Nature Communications, 2022. 13, 672
4. PNAS. 2023. 120, e2213154120
5. Trends. Biotechnol, 2022, 40, 1405-1414