(337bq) Novel Wet Adhesives Derived from Vibrio Cholerae Biofilm Adhesins

Adhesives that can function under the water have many important applications in industries such as fishing, underwater vehicles, and biomedical fields. However, designing effective wet adhesives is very challenging due to the presence of a hydration layer tightly bound with negatively charged surfaces commonly seen in the environment, which hinders the ability of the glue to interact with the real surface.

In a serendipitous discovery on Vibrio cholerae biofilm adhesins, we discovered a sequence of 57-amino acids (57-aa) with unique characteristics that is the major contributor to Vibrio cholerae adhesion to various abiotic surfaces, including glass and plastics, and biotic surfaces including lipid membranes. We developed and conducted multiple biochemical and biophysical in vitro characterization methods, such as fluorescence-based microbeads adsorption assay to quantitatively demonstrate the potential of the 57-aa as a wet adhesive. By varying length and sequences derived from the original 57-aa sequence, and modifying the lipid membrane composition, we identified key sequence features involved in 57-aa interactions with lipid membranes and abiotic surfaces. We constructed corresponding Vibrio cholerae mutants and tested their adhesion performance in biofilm settings, and collaborated with simulation groups to develop a data-explaining theory. After establishing a generic model for the 57-aa adhesion mechanism, we engineered for better wet adhesives based on the underlying science.

In conclusion, we demonstrate that a sequence of 57-aa derived from Vibrio cholerae and its variations could be used as bioactive glues to adhere to surfaces with different chemistries under the water. Our findings could pave the way for developing more effective wet adhesives with a broad range of applications.

Research Interests

Biomaterial, Medical device, Infectious Diseases, Formulation