(261a) Bio-Inspired Small Molecule Recognition for Point-of-Care Sensors

In the current clinical settings, the physicians mainly rely on the patient report to conduct opioid therapy and recovery plan, including drug screening and dose titration. Therapeutic drug monitoring (TDM) used in relapse prevention, maintenance, or withdrawal treatment in substance-related disorders is not established in clinical practice due to lack of efficient tool. To the best of our knowledge, a portable device for POC opioid detection has not yet been developed. This new detection method was inspired by the complementary base pairs mechanism. Modern organisms use nucleic acids to store and transfer genetic information utilizing the complementary base pairs called Watson−Crick (WC) and Hoogsteen base pairing. The nucleic acids are selectively paired through 2-4 intermolecular hydrogen bonds.It granted high molecule recognition for precisely constructing the complex DNA and RNA structure. Comparing with the planer Watson−Crick base pairing, Hoogsteen base pairing is to construct the complex non-double helix. The 3-dimensional hydrogen bond pattern of Hoogsteen base pairing offers an atomic-level molecular recognition and even higher stability against body environment variations. Inspired by this high selectivity and stability, we developed the pairing anchor composites could selectively adsorb the analyte molecule through a contrivable hydrogen pattern. The molecule-recognition interfaces on the flexible conducting polymer was served as a high-performance detection sensor for rapid and accurate measurement within fluid body samples without a pre-separation. The anchor molecule was immobilized on the electrode surface, with a Intermolecular Interaction-Enabled Small Molecule Recognition (iMSR), to design for small drug selective adsorption. We have successfully applied this method to the acetaminophen and fentanyl sensor.