(180e) A NOVEL Bioprocess Strategy for High Cell Density Cultivation of Probiotic Strain Limosilactobacillus Reuteri

Among various species of Lactobacillus had been explored as probiotic candidates, the Limosilactobacillus reuteri received much attention and successfully developed into commercial products for consumption by human and for other animals. The L. reuteri had been recognized with multiple beneficial effects especially related to gastrointestinal and immune system. Their heterofermentative ability also had been utilized for production of various organic acids and other bioactive compounds. However, the production of higher concentration of organic acids and other growth inhibitory factors during fermentation had been major downside for high cell density production of probiotics cells, including for L. reuteri. Numerous studies had utilized bioprocess strategies to improve the fermentation process to achieve high cell mass production in a cost effective platform. Therefore, in the present study, a novel strategy of removing lactic acid during the fermentation of L. reuteri had been investigated. This was the first report of utilizing anion exchange resins in the high cell density of cultivation of L. reuteri. The study had employed two weak-base anion exchange resins of Amberlite IRA 67 and IRA 96 to adsorb the accumulation of lactic acids. Both batch and fed-batch cultivations using the anion exchange resins had proven to increase the number of viable cells. The study reported that an externally integrated resin column system promoted high cell mass production compared to in-situ addition of resins. This resin solum had improved 71 times in viable cell count (3.89 × 1011 ± 0.07 CFU mL−1) had compared to control batch cultivations (5.35 × 109 ± 0.32 CFU mL−1). This was achieved as the resins adsorbed higher concentration of lactic acids from the fermentation broth. These results support the industrial use as an alternative to produce probiotics low-cost ingredients to obtain high biomass concentrations in bioreactors.