(105f) Maximizing the Biofuel Production Form Algae Using Four Element Strategy and Ultimate Economic Benefits

The Four Element Strategy (FES) was used to maximize biofuel production from algae strains and assess the associated economic benefits. The FES is based on considering the type of algal strains, concentration of input CO₂ (C_c,g), light intensity (L_I) energy, and nutrients requirements (Nitrogen (N) to phosphorus (P) ratio, R_N/P) achieve the maximum benefits . Two algal strains was used in the current study, encompassing slain algae Nannochloropsis sp. (Ns), and fresh water strain chlorella vulgaris (Cv). Response Surface Methodology (RSM) and Box Behnkin Design (BBD) combined with the biokinetic model was used to investigate the relation between C_c,g in the range0.03-20 %, R_N/P in the 1- 11) and L_I 100-400 µE m^-2 s^-1) and the corresponding CO₂ gas fixation rate (R_CO2, g L^-1 d^-1), biomass accumulation (X, g L^-1), and algal growth rate (µ, d^-1). The results revealed that the optimum productivity conditions are; L_I 100-270 µE m^-2 s^-1, C_c,g 0.03-15%, R_N/P 3.5 -11. The corresponding to a maximum R_CO2 , X and µ were 1 g L^-1 d^-1,.6 g L^-1, and 1.6 d^-1, respectively. The optimized conditions using the desirability function support that a R_CO2 of 1.2 g L^-1 d^-1, X of 1.8 g L^-1and µ of 1.7 d^-1, when the R_N/P,Cc,g , and L_I were 11, 4.5% and 200 µE m^-2 s^-1respectively. The N and P uptake rates for NS were reported at 85 mg L^-1 d^-1 and 17.1 mg L^-1 d^-1 compared with only 60.15 mg L^-1 d^-1 and 2.1 mg L^-1 d^-1 for Cv, respectively. The maximum B_YP of 1.47 g L^-1 d^-1 was achieved based on the optimum MCT conditions of C_c,g in the range 4-16%); L_I in the range 200-250 µE m^-2 s^-1, and R_N/P in the range 8-11 respectively. The total cost required to produce 1 L of biofuel was found to be 0.629 $, and the required cost to cover this cost is 0.138 $ and 0.375 $ for NS and Cv, respectively. The current study provide the guide way for large-scale process for MCT.