(19g) Mechanical Regulation of Cancer Cell Angiogenic Activity
AIChE Annual Meeting
Sunday, October 28, 2018 - 5:18pm to 5:36pm
Non-enzymatic glycation of collagen with different concentrations ribose was carried out to engineer mechanically tunable (same protein concentration) matrices. Highly invasive (MDA-MB-231) and non-invasive (MCF-7) breast cancer cells were encapsulated in these matrices of varying mechanics and cultured in the presence of serum-free media. 4 days post-fabrication, the spent media (containing secreted bioactive factors) were collected and concentrations of angiogenic molecules in the media were measured via Proteome ProfilerTM Human Angiogenesis Array (R&D Systems). Matrix stiffening altered angiogenic activity of MDA-MB-231 cells as manifested from enhanced secretion of pro-angiogenic (e.g. VEGF and angiopoentin-2) and reduced secretion of anti-angiogenic factors (e.g. TIMP-1 and thrombospondin-2). However, such drastic effects were not observed for MCF-7 cells indicating less dependency on matrix stiffness. These data suggest the existence of a potential mechanoregulatory circuit in controlling tumor vascularization. The impact of inhibiting mechanotransduction events on angiogenic activity of cancer cells will be discussed.
This study was supported by Alternatives Research and Development Foundation.