Quantification of Phospholipid Vesicle Degradation Kinetics Catalyzed By a Model Phospholipase
AIChE Annual Meeting
2017
2017 Annual Meeting
Student Poster Sessions
Undergraduate Student Poster Session: Food, Pharmaceutical, and Biotechnology
Monday, October 30, 2017 - 10:00am to 12:30pm
The morphologies of the GUVs were observed before and after adding enzyme and degradation kinetics were quantified by calculating the change in surface area of the GUVs. A threshold concentration of sPLA2 was discovered, beyond which the GUVs shrink significantly fast. More specifically, combinations of 1, 2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1, 2-dilinoleoyl-sn-glycero-3-phosphocholine (DLPC, 18:2) were used in this study.
It was found that above 1μg/ml of sPLA2, DPPC GUV degradation became obvious within the observation time (30 min). With the presence of the unsaturated DLPC, the threshold concentration of sPLA2 is reduced. The structures formed after degradation were also different for saturated and unsaturated lipids. While saturated lipids formed crystal-like structures, unsaturated lipids were seen momentarily shrinking and then relaxing back to a spherical shape. These results will help design a system for targeted drug delivery.