(197b) Intracellular Trafficking of Enzyme-Cleavable Peptide Amphiphiles
Recently, we showed enhanced intracellular controlled release and accumulation of the peptides by introducing endosome-specific enzyme cleavable linkers between the hydrophobic tail and peptide of the PA building blocks. In addition to enhanced accumulation, the delivery platform that we showed offers the ability to track the individual therapeutic and hydrophobic components in real time using fluorescent labels to better understand and manipulate intracellular drug trafficking. Thus, this platform is a superior nanomedicine tool for enhanced intracellular delivery and tracking of therapeutics.
In this study, we studied the trafficking of the peptides after endosomal enzyme cleavage. We synthesized the peptide amphiphiles, as described in our previous study. In this follow-up study, we added endosomal escape groups to the peptides. Different organelle targeting peptides were selected and their co-localization was studied with different organelle fluorescent labels.