(347g) Analysis Of Exahled Breath For Diagnosis Of Tuberculosis

Analysis of exhaled breath promises to be a powerful tool for noninvasive diagnosis. Some carbonyl compounds have been reported as metabolic markers of Tuberculosis (TB). However, so far there is no established protocol for the analysis of carbonyl compounds in exhaled breath. We previously reported a preconcentrator approach for capture of carbonyl compounds in exhaled breath. However, the preconcentrator approach requires a long time for exhaled breath flowing through the preconcentrator and it is difficult to deal with infectious exhaled breath samples of TB patients. Here we reports a simple sampling chip with reactive aminooxy coating for the capture of carbonyl compounds in exhaled breath. The reactive silicon chip has thousands of micropillars in a microfluidic channel. The reactive chips were fabricated on 4"-silicon wafers. Deep reactive ion etching (DRIE) was used to create the channels and micropillars with a height of 100 µm. The micropillar surfaces were coated with a reactive aminooxy compound for trapping trace carbonyl compounds by means of oximation reactions. The captured ketone- and aldehyde adducts were eluted by ethanol and were directly analyzed by gas chromatography-mass spectrometry (GC-MS) and Fourier transform-ion cyclotron resonance (FT-ICR) mass spectrometry (MS). The results indicate that this method of breath analysis can be used for diagnosis of TB patients.