(181d) Liquid-Continuous Impinging Stream Used for Cell Disruption and Its Flow Field Simulation

Guo, J., Wuhan Institute of Technology
Jian, G., Wuhan Institute of Technology
Ding, Y., Wuhan Institute of Technology
Yang, X., Wuhan Institute of Technology
Zhou, Y., Wuhan Institute of Technology
WU, Y., Wuhan Institute of Technology

It was found that liquid-continuous impinging stream (LIS) might promote micromixing and had strong pressure fluctuation due to the intensive collision, squeezing and shearing among opposing stream micells. The shearing strength of LIS and its drag force to inert particles have potential applications, for example, for cell disruption. Cell disruption is a very important step for biomass down-stream processing and extraction of effective compounds from Chinese herbs. In water treatment, it may apply for removal of algae, fungi and bacteria. In this paper, the method of yeast cell disruption by a self-designed vertical impinging stream reactor was introduced, and the effects of impinging speed, impinging time and adding medium quantity on the ratio of cell disruption were investigated. The experimental results show that under the conditions of 1200 r/min of motor speed, 7 h of impinging time and 200 g of adding agent, the ratio of cell disruption can reach 75.8% by the vertical impinging streams reactor. A numerical simulation was also be carried out for flow filed of liquid-continuous impinging stream, and its pressure field and velocity field were studied. This method is expected to play an important role in cell disruption with hydraulic power, and also to be beneficial to further develop the theory of liquid-continuous impinging streams.