(418g) Genetic Engineering of Escherichia Coli K-12 for the Development of Nano-Tubes (Fimbriae=Pili) for Effective Drug Design

One of the major breakthrough at the present scientific and engineering world would be the development of specific molecular structure oriented drug and enzyme -designs. However their techniques are very laborious and costly. In the present paper the author will discuss the method in which the specific drug application, specially the immune response based micro-nano-tubes (fimbriae=pili) can be developed through which the drug applications could be faster.

The microtubules were the elongated surface appendages (fimbriae=pili) of Escherichia coli and other microbial species, Fig.1-5. They are specific and are used particularly to stimulate immune response and cell lysis and removals of toxic soil contaminants (aromatics=penols) in the process of bio-transformations. A case study causing diarrhea to Balb/c mice was considered to validate the process of specificity to protect mice against 026:EPEC (enteropathogenic Escherichia coli) fetal diarrhea. Genetically engineered E.coli K-12 was made for these specific drug, immune and removal activities.

The development of such transgenic hybrid, genetically engineered E.coli K-12, C600 Yale auxotrophic strains were made by transfer of extra chromosomal plasmid-DNA of the donors, who are identified causing diarrhea, and supports tumor growths, either by the support of plasmid vectors (pBR322, pSU101, pDG1 or pNB) in vitro or in vivo by classical co-conjugations( multiple conjugations with donors and recipients) under selective pressure of auxotrophic E.coli K-12 pheno--genotypic and antibiotic drug resistant activities. The hybrid strains and their surface antigenic nano-tubes (fimbriae=pili) of these bacterial groups were then isolated and were used for the specific drug activities. In this case the same method was used for protecting mice against fetal diarrhea. Data reveled that the specificity to prevent infection (diarrhea, drug release tumor growths inhibition) or biodegradability= biotransformation of soil can be enhanced through this unique technique as cost-effective ecofriendly applications. For the production of specific drug designed molecules (nano-molecules, nano-tubes) the applications of genetically engineered bacterial methods would be essentially a challenging attempt.