Direct Monitoring of Oil Production in Individual Microalgae in Situ by Raman Spectroscopy

Solar Topical
2009 AIChE Annual Meeting
AIChE Annual Meeting
November 11, 2009 - 7:00pm
Microalgae, producing oils that can be converted into biodiesel, are one of the promising candidates for the next generation of viable fuel to help solve the global energy crisis. However, the recent booming of research on microalgae, so far appears not benefiting from the booming of modern high-throughput, high-resolution, real-time, and in vivo biophysical techniques. Traditionally, these microalgae were treated in bulk, lyophilized or in extracted forms, making it impossible to assess the information on fundamental biological processes in the single-cell or sub-cellular level. In this work, we focus on in situ, in vivo and label-free Raman characterizations of modeled algal lipids, extracted algal oil, and most importantly, single living algae (Figure 1). Our study has demonstrated a label-free and direct method to obtain quantitative information of chain length and degree of unsaturation of the oil produced inside algae. It also connects with the important issues of the cloud point and the quality of algal biodiesel. Our single-cell, real-time, and in vivo study of algae, with various lipid-triggering mechanisms, enables the possibility of researching and engineering of the best conditions and the best species for algal growth and oil production. Figure 1. A single Neochloris oleoabundans algal cell is held by an optical trap and its Raman spectrum is obtained. Black arrows are pointing at the Raman bands of oil identification. A dashed arrow is pointing at the Raman band of unsaturation (C=C bonds) of the oil inside the alga.&'
Professional Development Hours
0.4 PDHs
You will be able to download and print a certificate for these PDH credits once the content has been viewed. If you have already viewed this content, please click here to login.

Would you like to access this content?

No problem. You just have to complete the following steps.

You have completed 0 of 2 steps.

  1. Log in

    You must be logged in to view this content. Log in now.

  2. Purchase Technical Presentation

    You must purchase this technical presentation using one of the options below.
    If you already purchased this content recently, please click here to refresh the system's record of ownerships.


Credits 0.5 Use credits
List Price $25.00 Buy now
AIChE Members $15.00 Buy now
AIChE Undergraduate Student Members Free Free access
AIChE Graduate Student Members Free Free access
Related Topics: 
Skill Level: