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Systems Metabolic Engineering of Corynebacterium Glutamicum for Production of Bio-Based 1,3-Propanediol from Lignocellulose

Chen, Z., Tsinghua University
Huang, J., Tsinghua University

1,3-Propanediol is an important chemical which is mainly used as monomer for the synthesis of valuable polymers. In this study, Corynebacterium glutamicum is systematically engineered for the high production of 1,3-propanediol from lignocellulosic sugars, including glucose, xylose and arabinose, etc. First, the heterologous modulars for glycerol and 1,3-propanediol synthesis were introduced and combinatorially optimized based on promoter engineering and enzyme screening. Next, the modulars for xylose and arabinose transport and degradation were imported. The fluxes to downstream pathway of glycolysis and competing products (acetate, lactate) were blocked or down-regulated. Furthermore, the sugar transprot system and the cofactor regeneration systems were optimized. The final engineered strain was able to produce more than 100 g/L 1.3-propanediol from glucose, xylose or their mixtures. This is an important step towards the enomical bio-production of 1,3-propanediol for polymer industry.