(27bn) Engineering of Tunable Cell Network with Endogenous Template Activation of Non-Excitable Cells

Neurodegenerative diseases refer to a group of progressive disorders that gradually affect the structure and function of the brain and nervous system, leading to the gradual loss of cognitive and physical abilities. These diseases are characterized by the degeneration and death of nerve cells, resulting in a decline in memory, movement, and coordination. Examples of neurodegenerative diseases include Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, and amyotrophic lateral sclerosis (ALS). Currently, there is no cure for most of these disorders.

In this study, an engineering approach was employed to construct a minimal neuron by dividing neurons into essential building blocks and incorporating inanimate materials such as proteins and lipids. The CRISPR activation technique was applied to activate endogenous templates in non-excitable cells, recreating the neuron's ability to communicate between cells and achieving a working binary response that is essential for information processing. The ultimate goal of the study is to gain a better understanding of how the brain works by adding components to the basic neuron unit.

Therefore, this study provides a platform and potential treatment for understanding the mechanism of neurodegenerative disorders. By developing a better understanding of the underlying mechanisms of these diseases, this research may contribute to the development of effective treatments and therapies.