(570f) 3D-Bioprinting of Spatially Organized Bacterial Microcosmoi Using Chaotic Flows
Our group previously reported the development of a 3D-printing technique (chaotic bioprinting) using a Kenics static mixer to produce alginate-based and agar-based fibers exhibiting internal lamellar microstructures. In this contribution, we use chaotic bioprinting protocols to create fine-scale bacterial microcosmoi. This straightforward approach allows us to place various bacterial strains in these constructs to analyze how their spatial distribution may affect their social behavior and/or survival abilities. We demonstrate that these biological microsystems can exhibit a competitive dominance as a function of culture time, the development of hypoxic regions, and the degree of interface shared between the microcolonies.
Chaotic bioprinting enables the printing of cell-laden constructs with fine-scale deposition patterns. We envision that this technique will contribute to the development of more complex poly-bacterial microcosmoi, such as gut-microbiota models.