(535h) Two Experimental Protocols for Accurate Measurement of Gas Component Uptake and Production Rates in Bioconversion Processes
One protocol is for depressurized (i.e., system pressure below 1 atm) batch systems, where N2 is used to repressurize the system to 1 atm before sampling; the other is for continuous operations, where He is used as tracer or internal standard to obtain accurate measurement of the off-gas flow rate. Both abiotic systems and biotic systems with an aerobic methanotroph were used to demonstrate the effectiveness of the proposed protocols and calculation procedures in ensuring accurate consumption and production rate measurements of gas components. The accuracies of the gas component measurements based on both protocols were assessed through percentage error when the true values are known, or through total carbon balance when the true values are unknown. The proposed protocols and associated calculation procedures are easy-to-implement, do not require specialized equipment, and are generally applicable to various bioconversion processes where gas components are involved.