(117d) Multimodal Study of Biological Corona Structure and Dynamics on Single-Walled Carbon Nanotubes
AIChE Annual Meeting
2019
2019 AIChE Annual Meeting
Nanoscale Science and Engineering Forum
Carbon Nanomaterials Graduate Student Award Session
Monday, November 11, 2019 - 1:21pm to 1:38pm
We present multimodal characterization of (i) the protein corona composition on polymer-SWCNTs in relevant biofluids, (ii) the structural conformation of the solubilized polymer-SWCNT complexes, and (iii) the kinetics of protein adsorption to the polymer-SWCNTs in solution. Single-stranded DNA-wrapped SWCNTs (ssDNA-SWCNTs) are chosen as the polymer-SWCNTs of interest due to their broad relevance in sensing4, neuro-imaging5, and chirality sorting6, though this platform is extendable to other nanoparticles. We determine the protein corona composition by a selective adsorption assay with characterization by protein mass spectrometry in both human blood plasma and cerebrospinal fluid. We next probe the morphology of ssDNA-SWCNT complexes using biological small angle x-ray scattering (BioSAXS). Differences in corona morphology are observed depending on solution ionic strength, ssDNA length, and exposure to key corona proteins. To characterize the dynamic exchange in the SWCNT corona phase, we develop a multiplexed fluorescence assay that enables real-time tracking of biomolecule adsorption and desorption events. Studies are conducted with systematic variation of the molecular entities (ssDNA, protein) and solution conditions, whereby binding profiles inform a kinetic model of the SWCNT system under exposure to different biological conditions. The binding kinetics are compared against an orthogonal platform monitoring solvatochromic shifting of the near-infrared fluorescent SWCNT spectrum as a proxy for SWCNT corona perturbations. The work presented herein develops an understanding of the fundamental corona exchange mechanism, contextualizes the nature of the ligand exchange process, and provides insight into performance of these SWCNT-based systems in biologically relevant, protein-rich conditions such as human blood plasma and cerebrospinal fluid.
References
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