(636d) A Way for Controlling Levan Nanoparticles Production Andparticle Size Distribution
AIChE Annual Meeting
2018
2018 AIChE Annual Meeting
Nanoscale Science and Engineering Forum
Self-Assembled Biomaterials
Thursday, November 1, 2018 - 9:45am to 10:00am
Levan nanoparticles are produced via the self-assembly of a polyfructose
biopolymer produced by some specific microorganisms.
However, the use of microorganisms for biopolymer production requires a deep
control of many variables such as culture medium agitation, speed, oxygen…Therefore,
cell-free systems, which use the isolated enzyme, have been proposed as a feasible
alternative to overcome these drawbacks and to improve the uniformity of polyfructose
in terms of molecular weight, and therefore of the levan nanoparticles formed by the
polyfructose self-assembly.
In this work polyfructose is prepared by the cell-free polymerization reaction
and the effect of the different sugar monomers has on the particle size, polymer
molecular weight and the polymer production rate is described.
Results show that particle size can be modified (from 150 nm to 250 nm) by
controlling the amount of sucrose and lactose in the culture medium. Furthermore, the
addition of different sugars modifies the branching and therefore the polymer properties.
Finally, our results indicate that the addition of different sugars would permit the
synthesis of levan nanoparticles à-la carte depending on the future use.
biopolymer produced by some specific microorganisms.
However, the use of microorganisms for biopolymer production requires a deep
control of many variables such as culture medium agitation, speed, oxygen…Therefore,
cell-free systems, which use the isolated enzyme, have been proposed as a feasible
alternative to overcome these drawbacks and to improve the uniformity of polyfructose
in terms of molecular weight, and therefore of the levan nanoparticles formed by the
polyfructose self-assembly.
In this work polyfructose is prepared by the cell-free polymerization reaction
and the effect of the different sugar monomers has on the particle size, polymer
molecular weight and the polymer production rate is described.
Results show that particle size can be modified (from 150 nm to 250 nm) by
controlling the amount of sucrose and lactose in the culture medium. Furthermore, the
addition of different sugars modifies the branching and therefore the polymer properties.
Finally, our results indicate that the addition of different sugars would permit the
synthesis of levan nanoparticles à-la carte depending on the future use.