(607f) Biomechanics Study of Endothelial Cellular Membranes Under Hypo-Osmotic Challenge
AIChE Annual Meeting
Thursday, November 1, 2018 - 9:30am to 9:48am
We find that hypo-osmotic swelling results in significant stiffening of the membrane region of endothelial cells, without a change in membrane tension/membrane-cytoskeleton attachment. Furthermore, depolymerization of F-actin in the cytoskeleton, which, as expected, results in a dramatic decrease in the cellular elastic modulus of both the membrane and the deeper cytoskeleton, indicating a collapse of the cytoskeleton scaffold, does not abrogate swelling-induced stiffening of the membrane. Instead, this stiffening is enhanced. We propose that the hypo-osmotically induced membrane stiffening should be attributed to an increase in hydrostatic pressure that results from an influx of solutes and water into the cells. Most importantly, our results suggest that increased hydrostatic pressure, rather than changes in membrane tension, could be responsible for activating volume-sensitive mechanisms in hypo-osmotically swollen cells.