(188z) Development of a Modular Pathway Optimization Toolbox for Synechococcus Elongatus PCC 7942

Balancing the burden of heterologous pathway expression with the need for robust cellular metabolism has always been an underlying principle for metabolic engineering projects. Here we present a cost and functionality-optimized pathway integration vector system for the cyanobacterial host, Synechococcus elongatus PCC 7942. This isocaudomer-based platform enables transcriptional and translational optimization of heterologous pathways in vivo. Part characterization and preliminary efforts towards a proof-of-principle alkaloid production study will be presented.