(85f) Cancer Cell Hyperactivity and Membrane Dipolarity Monitoring Via Raman Mapping of Interfaced Graphene: Towards Non-Invasive Cancer Diagnostics
- Conference: AIChE Annual Meeting
- Year: 2017
- Proceeding: 2017 Annual Meeting
- Group: Topical Conference: Nanomaterials for Applications in Energy and Biology
- Time: Monday, October 30, 2017 - 10:05am-10:30am
Ultra-sensitive detection, mapping and monitoring of the activity of cancer cells is critical for treatment evaluation and patient care. Here, we demonstrate that a cancer cellâs glycolysis-induced hyperactivity and enhanced electronegative membrane (from sialic acid) can sensitively modify the second-order overtone of in-plane phonon vibration energies (2D) of interfaced graphene via a hole-doping mechanism. By leveraging ultrathin grapheneâs high quantum capacitance and responsive phononics, we sensitively differentiated the activity of interfaced Glioblastoma Multiforme (GBM) cells, a malignant brain tumor, from that of human astrocytes at a single-cell resolution. GBM cellâs high surface electronegativity (potential ~310 mV) and hyperacidic-release induces hole-doping in graphene with a 3-fold higher 2D vibration energy shift of approximately 6Â±0.5 cm-1 than astrocytes. From molecular dipole induced quantum coupling, we estimate that the sialic acid density on the cell membrane increases from one molecule per ~17 nm2 to one molecule per ~7 nm2. Further, graphene phononic response also identified enhanced acidity of cancer cellâs growth medium. Grapheneâs phonon-sensitive platform to determine interfaced cellâs activity/chemistry will potentially open avenues for studying activity of other cancer cell types, including metastatic tumors and characterizing different grades of their malignancy.