(341f) Engineering Studies of the Effect of pH and Temperature on the Adsorbent Surface Interaction for the Anion-Exchange Chromatographic Separation
AIChE Annual Meeting
2017 Annual Meeting
Chromatographic Separations and SMB
Tuesday, October 31, 2017 - 2:10pm to 2:30pm
This presentation explores a fundamental case of separation involving amino acids on a strong anion-exchange resin. The pH and temperature were increased and the retention time amino acids on a Sepharose Q Fast Flow column increased. When analyzing the data obtained from the chromatograms, the moments method by Schneider and Smith was employed. From the moments analysis, the adsorption and desorption rates were calculated. The adsorption and desorption rate were fitted to an Arrhenius expression to quantify the activation energy. The main outcomes of this investigation are that the retention increases with increasing pH and temperature. Along with that the adsorption rate was determined to be a diffusive phenomenon. Another important takeaway from this paper is that the adsorption rate has a positive activation energy and the desorption has a negative activation energy. This information can then be used to simulate the optimum pH and temperature conditions for the separation.