(264b) Direct Succinic Acid Production from Non-Hydrolyzed Biomass Using Sequential Solid-State and Slurry Fermentation with Mixed Fungal Cultures

Alcantara, J., Western Michigan University
Hughey, L., Western Michigan University
Shields, S., Western Michigan University
Mondala, A., Mississippi State University
This study explores a new route for directly producing succinic acid from lignocellulosic biomass via consolidated bioprocessing technology employing lignocellulolytic and acidogenic fungal co-cultures. The consolidated bioprocessing technology involves two-stages: a solid-state fermentation pre-culturing stage followed by slurry fermentation stage. During the solid-state pre-fermentation stage, the filamentous fungi Aspergillus niger and Trichoderma reesei were co-cultured in a nitrogen-rich substrate (e.g., soy hull) to produce cellulolytic enzymes for degrading biomass cellulosic and hemicellulosic fractions to fermentable sugars. The lignolytic fungus Phanerochaete chrysosporium was grown separately on carbon-rich wood chips to induce lignolytic enzymes that degrade lignin, rendering the biomass more susceptible to cellulase attack. The solid-state pre-cultures were then combined in a slurry fermentation culture to achieve simultaneous enzymatic cellulolysis and succinic acid production. Succinic acid was generated with a maximum titer of 49.0 g/L and yield of 19.6 g succinic acid per 100 g total dry biomass substrate after 36 h of slurry fermentation. This approach is a promising alternative to conventional bacterial succinic acid production due to its minimal substrate pretreatment requirements, which could potentially reduce production costs.


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