(191cd) Colorimetric Virus Detection Using Gold Nanoparticle Aggregation
Our model virus porcine parvovirus (PPV), can be non-specifically adsorbed to AuNPs to form a virus corona. The AuNPs coated in virus have a measurable aggregation when osmolyte is present as compared to AuNPs coated in model protein bovine serum albumin (BSA) and exposed to osmolyte. Osmolytes are natural, organic compounds that are found in many organisms. Our lab has shown that osmolytes preferentially flocculate both an enveloped and non-enveloped virus but not model proteins. In order to create a quick, sensitive, inexpensive and portable method for detecting the presence of viral particles, we are employing the preferential flocculation of virus particles with osmolytes in the presence of AuNPs.
To distinguish the aggregation of AuNPs with virus solutions from that with BSA, solutions at various ionic strengths and with different concentration of osmolyte solutions was explored. Glycine at 1 M can be used to distinguish the AuNP aggregation of coronas formed from PPV and BSA. Lower ionic strength buffer used for corona formation give the largest difference between BSA and PPV. In addition, the key parameters that increase the sensitivity and decrease the limit of detection of PPV will be discussed. This unique method can easily be transferred to other virus systems and be used in the detection of norovirus on cruise ships.