(398d) Modeling for Complex Interactive Behaviors Among Enterococcus Faecalis Donor and Recipients in Biofilms
We have formulated a mathematical stochastic model that captures the dynamic interaction and responses of both recipients and donors in the entire population. Both types of cells were embedded inside the biofilm and were influenced by both extracellular cCF10 and iCF10. The model also attempts to capture the plasmid spreading effect through which some recipients are converted into new donors. The biofilm is formulated as a 2D model and is composed of both donors and recipients. This model accounts for the following effects: 1) molecular interaction between intracellular quantities prior to conjugation, 2) mechanistic genetic regulation of the conjugations and the corresponding outcomes, and 3) population level, signal peptides and donor/recipient population in the biofilm and recipients. We anticipate that the initial locations of donors and recipients and the availability of signaling molecules within the biofilm can affect the plasmid transfer between cells. A higher donor concentration means a higher number of available plasmids to transfer but also leads to more production of iCF10, suppressing the conjugation to avoid any wasteful plasmid transfer. This model helps to evaluate these competitive effects in different scenarios and thus provides a full set of conditions at which some factors can be more dominant than others and has the potential to fully capture all the different effects from both donors and recipients. Understanding the balancing between different signaling mechanisms in this system may shed light on new ways of controlling the spread of antibiotic resistance.