(338b) A Lab-on-a-Chip Platform of Mono-and Poly-Microbial Biofilms for High-Throughput Downstream Applications
METHODS: We have developed a novel lab-on-a-chip platform of mono-and poly-microbial biofilms of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans for high-throughput antimicrobial screening. The nano-biofilm chip (nBioChip) is robotically printed, robustly handled and scanned using a standard microarray reader. The platform is â??trulyâ? high-throughput and serves as a rising alternative to conventional modes of drug screening.
RESULTS: The biofilm chip consisted of identical nano-biofilms embedded in a hydrogel and exhibited resistance to washing steps involved in the drug screening assay. The biofilm, although nano-scale in nature, demonstrated i) the morphology and three-dimensional architecture of a macroscopic biofilm; ii) elevated drug resistance to antibiotic treatment; and iii) synthesized idiosyncratic exo-polymeric matrix material of a true biofilm. Based on our results, the nBioChip can generate reliable high-throughput antimicrobial susceptibility testing (HT-AST) in 12-18 h. The chip serves as a proof-of-concept platform for high-throughput drug screening against any microorganism and furthers the understanding of microbial interactions in mixed-species communities.