(98au) Suspension Displaying On Through-Hole Array Membranes

Microbeads are currently being used in a variety of biomedical assays due to inherent benefits such as the ease of separation, high surface-to-volume ratio, and flexible modification of targets. Although several platforms have been suggested for analyzing the results of bead-based assays, there has been few detection platforms suitable for portable use. The reason for this absence is the difficulty of incorporating portability into decoding systems for suspension arrays without the use of complicated and cumbersome optical set-ups. Here, we present a decoding unit for suspension processing that uses a micro-machined through-hole array membrane. Based on the bead sorting and capturing capability of the membranes, the displaying unit successfully decodes the information from mixed poly-disperse suspension arrays. Also, a quantitative guide for device design is provided based on the study of the hydrodynamic interaction between the beads and the through-hole membrane. As a proof-of-concept, the displaying unit is used as a detection platform for multiplexed suspension ligase detection reaction (LDR).