(694g) Nonlinear Direct Inverse Method: A Way to Combine Nonlinear Calibration with Isotherm Determination

Nonlinear direct inverse method: a way
to combine nonlinear calibration with isotherm determination

Monzure-Khoda Kazi
, Bijan Medi, Mohammad Amanullah*

School
of Chemical and Biomedical Engineering
Nanyang Technological University

62
Nanyang Drive, Singapore 637459

Singapore

(aman@ntu.edu.sg)

This work
addresses a way to combine nonlinear calibration with isotherm determination. In
this method, like classical inverse method, experimental elution profiles are
compared with the results of a detailed model that accounts for nonlinearity in
equilibrium, axial dispersion, and mass transfer kinetics. However, unlike classical
inverse method, the calibration of detector is carried out simultaneously with isotherm
determination thereby reducing cost and saving time. In this study no
limitation is imposed on the linearity of the detector signal or on the
overlapping of elution profiles in case of enantiomers. The method has been
experimentally verified for the separation of a mixture of guaifenesin
enantiomers over a wide range of operating parameters.