(489d) Analysis of Intracellular Movement Mechanism of GWBs Using High Resolution Particle Tracking

GW bodies (GWBs) are discrete cytoplasmic foci which play important role in microRNA induced post-transcriptional regulation of gene expression. GWBs are rich in proteins that are involved in microRNA induced regulation, mRNA degradation, translational repression, mRNA surveillance and RNA-mediated gene silencing. GW bodies assemble, disassemble and aggregate dynamically in cells. Analysis of intracellular movement of GWBs is essential to reveal the interactions they develop with their environments, especially with main cellular scaffolding?cytoskeletal filaments, which may play essential role in its cellular functions. This study analyzed trajectories of GWBs movements, and revealed the motion types of GWBs by combining high resolution particle tracking technique and computational simulation results of statistical characterization of different motion types. Our results quantitatively analysis the distribution of motion types among GWBs, and further revealed the relationship between GWBs movement and cytoskeleton. This study investigated the mechanism of intracellular movements of GWBs, and also provided a convenient method to study movement mechanism of other dynamic intracellular protein foci/vesicles.