(291ae) Minimizing the Surface Effect on Pcr in Pdms-Glass Chips by Dynamic Passivation

Authors: 
Hua, Z., University of Michigan
Srivannavit, O., University of Michigan
Ozel, A. B., University of Michigan- Ann Arbor
Gulari, E., University of Michigan


Dynamic passivation of surfaces of PDMS-glass PCR chips using polyethylene glycol (PEG) or polyvinylpyrrolidone (PVP) was achieved in a conventional thermocycler. The effects of molecular weight of the polymers and the polymer concentration in PCR mixture on surface passivation were investigated. Efficiency of the dynamic passivation follows the order: PVP10000 > PVP55000, PEG8000> PEG10000> PEG400. 0.4% and 0.025% (w/v) are the suitable final polymer concentrations in PCR mixture. Competitive adsorption could be the principal activity while the effect of polymers on Taq polymerase is a minor function during the PCR.

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