(534b) Application of Protocatechuic Acid-Based Deep Eutectic Solvent for Utiliztion of Engineered Biomass
AIChE Annual Meeting
2022 Annual Meeting
Forest and Plant Bioproducts Division
Poster Session: Novel Products from Forest and Plant Biomass
Wednesday, November 16, 2022 - 3:30pm to 5:00pm
To efficiently valorize lignin and achieve a sustainable biorefinery process, genetically engineered sorghum bagasse with altered lignin monomers was pretreated by a novel protocatechuic acid-based deep eutectic solvent (DES). We synthesized the DESs with protocatechuic acid (PCA) and choline chloride (ChCl) and applied this solvent to fractionate sorghum bagasse. The transgenic sorghum bagasse having protocatechuate incooperated lignin was used as a feedstock in this study. Additionally, three-component DESs containing ChCl, PCA and water or ethylene glycol were formed and tested. The fractionated lignin from the black liquor was subjected to hydrothermal treatment to recover protocatechuic acid, and the cellulose-rich pulp was enzymatically hydrolyzed. Structures of isolated lignin before and after hydrothermal treatment were characterized by two-dimensional heteronuclear single quantum (2D HSQC) NMR and gel permeation chromatography, while the liquid products of hydrothermal treatment were analyzed by gas chromatography-mass spectrometry (GC-MS).