(165h) Pulmonary Functionality Changes Following Titanium Dioxide Particulate Exposure and Antioxidant Protection
AIChE Annual Meeting
Monday, November 14, 2022 - 3:30pm to 5:00pm
Titanium dioxide nanoparticles are widely used in industrial and consumer products including paints, plastics, pharmaceuticals, cosmetics, and food products. Large production of this nanoparticle and other engineered nanoparticles are a cause for concern of occupational exposure through inhalation and dermal contact. TiO2 nanoparticle toxicity has been studied extensively in dermal models, however, respiratory effects and oxidative damage pathways remain a concern.
This study aims to characterize oxidative stress response in human pulmonary cell line A549 following isolated and repeated exposures to titanium dioxide nanoparticles. Size, charge, and morphology of TiO2 nanoparticles were characterized using DLS, zeta potential, and SEM prior to exposure. Post-exposure investigation included cell viability by MTT assay and physiological response parameters including IL-8 secretion, apoptosis execution determined by caspase 3 activation, ratio of reduced and oxidized glutathione, and quantification of reactive oxygen species. Further, antioxidant pretreatment is investigated to supplement decreased antioxidant capacity and reduce extent of functionality impairment.