(413d) Understanding of Metabolic Pathways in Pseudomonas Putida for Biosynthesis of PHA Using Sugar, Glycerol, Benzoate, and Their Combinations

Efficient utilization of all available carbons from lignocellulosic biomass is critical for economic efficiency of a bioconversion process to produce renewable bioproducts. However, the metabolic phenotypes enabling bacteria such as Pseudomonas to utilize mixed carbon sources to generate energy and stored bioproducts remain poorly understood. In this study, Pseudomonas putida KT2440 was employed to investigate the polyhydroxyalkanoates (PHA) production from mixed carbon source of glucose/benzoate or glycerol/benzoate. A multi-omics approach involving label-free proteomics and NMR metabolomics was taken to compare the metabolic profiles of different mixed carbon sources. Results showed different patterns of reductant generation and fatty-acid metabolism pathways with different mixed carbon sources, which resulted in enhancing or inhibiting biomass and PHA productions. Overall, our study provides new insights into the metabolic architecture of P. putida with different mixture carbon substrates and reveals the potential regulatory metabolic nodes that may guide further system biology design to improve the PHA production.