(322c) Real Time Dynamic Optimisation for Enhanced Product Quality in Bioprocesses
AIChE Annual Meeting
Tuesday, November 17, 2020 - 8:30am to 8:45am
The performance of such processes is assessed both for productivity and product quality. The latter is a broad concept that includes various aspects, amongst which product functionality. In mAb production, glycosylation percentage is one of the key indicators to measure cell culture quality performance. Glycosylation is a post-translational modification of mAbs that affects their stability and efficacy as therapeutics. As such, it is considered a Critical Quality Attribute (CQAs) under the Quality by Design (QbD) framework and needs to be controlled within a pre-specified range . There have been various studies on the improvement of mAb glycosylation intervening both at process- ,  and genetic- level , . However, the complexity of the system and its underlying interactions make process optimisation difficult, with optimality unlikely to be achieved solely by experimental studies. This is because strategies to improve mAb glycosylation can enhance mAb glycosylation but, at the same time, reduce cell growth and, thus, mAb concentration at harvest.
In this work we use an Ordinary Differential Equation (ODE) model as digital-twin for an IgG-producing CHO cell culture system . The model  comprises three main components; namely an unstructured model to describe cell growth, a reduced kinetics model for the intracellular nucleotide sugar donor (NSD) synthesis and a mAb glycosylation model. The mAb glycosylation model as considered in this work, comprises four continuous stirred tank reactors (CSTRs) using the reaction network presented in del Val et al.. We formulate and solve an exhaustive dynamic optimisation experiment to identify feeding regimes for galactose and uridine under which the amount of galactosylated antibody is maximised. The performance of every optimisation formulation is assessed based on: (a) achieved galactosylation percentage, (b) productivity, (c) culture viability and (d) experimental applicability. Towards an effort to enhance USP automation, measured process disturbances are introduced in the problem formulation. In this respect, the most successful scenarios are used as a basis for the design of a real time dynamic optimisation strategy as a means to achieve the optimal operational policy . The system is re-optimised online and based on the effect of the disturbances to the process, adapts and calculates the optimal input trajectories. In this work these are validated in closed-loop against the process model.
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